S100B ELISA Kit (Human)

CAT: 0247-OKEH02888-96WSize: 96 WellDry Ice: NoHazardous: No
CAT#:0247-OKEH02888-96WSize:96 Well
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CAS Number
7732-18-5
Gene Name
S100 calcium binding protein B
Gene Aliases
NEF; protein S100-B; S100; S100 calcium-binding protein B; S100 calcium-binding protein, beta (neural) ; S-100 calcium-binding protein, beta chain; S-100 protein beta chain; S-100 protein subunit beta; S100-B; S100beta.
Gene ID
6285
Accession Number
NP_006263.1
Reactivity
Homo sapiens, Human
Target
Protein S100-B
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology S100B ELISA Kit (Human) (OKEH02888) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for S100B has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for S100B is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample S100B captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
Detection Range
15.6-1000pg/mL
Recovery
<= 6.4%
Sensitivity
7.8 pg/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
10 kDa
Specificity
Natural and recombinant Human Protein S100-B
Protein Length
92
NCBI Gene Symbol
S100B
Protein Name
P04271
Gene Name URL
S100B
Nucleotide Accession Number
NM_006272.2

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