S100B ELISA Kit (Mouse)

• Gene Name:

  S100 protein, beta polypeptide, neural

• Gene Aliases:

  AI850290; Bpb; protein S100-B; S100 calcium-binding protein B; S-100 protein beta chain; S-100 protein subunit beta.

• Gene ID:

  20203

• Accession Number:

  NP_033141.1

• Reactivity:

  Mouse, Mus musculus

• Target:

  Protein S100-B

• Type:

  ELISA Kit

• Applications:

  Enzyme-linked Immunosorbent assay-Sandwich

• Assay Principle:

  Aviva Systems Biology S100B ELISA Kit (Mouse) (OKEH00537) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Mouse S100B has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. After washing, a biotinylated detector antibody specific for Mouse S100B is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Mouse S100B captured in well.

• Assay Protocol:

  Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

• Sample Type:

  Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids

• Detection Range:

  15.6-1000pg/mL

• Recovery:

  <= 5.9%

• Sensitivity:

  7.8 pg/mL

• Reconstitution:

  Store as indicated in product manual.

• Molecular Weight:

  11 kDa

• Notes:

  Formerly GWB-DA1ZH8

• Specificity:

  Natural and recombinant Mouse Protein S100-B

• Protein Length:

  92

• NCBI Gene Symbol:

  S100b

• Protein Name:

  P50114

• Gene Name URL:

  S100b

• CAS Number:

  7732-18-5

• Nucleotide Accession Number:

  NM_009115.3