S100B ELISA Kit (Mouse)

CAT: 0247-OKEH00537-96WSize: 96 WellDry Ice: NoHazardous: No
CAT#:0247-OKEH00537-96WSize:96 Well
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CAS Number
7732-18-5
Gene Name
S100 protein, beta polypeptide, neural
Gene Aliases
AI850290; Bpb; protein S100-B; S100 calcium-binding protein B; S-100 protein beta chain; S-100 protein subunit beta.
Gene ID
20203
Accession Number
NP_033141.1
Reactivity
Mouse, Mus musculus
Target
Protein S100-B
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology S100B ELISA Kit (Mouse) (OKEH00537) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Mouse S100B has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. After washing, a biotinylated detector antibody specific for Mouse S100B is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Mouse S100B captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
Detection Range
15.6-1000pg/mL
Recovery
<= 5.9%
Sensitivity
7.8 pg/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
11 kDa
Notes
Formerly GWB-DA1ZH8
Specificity
Natural and recombinant Mouse Protein S100-B
Protein Length
92
NCBI Gene Symbol
S100b
Protein Name
P50114
Gene Name URL
S100b
Nucleotide Accession Number
NM_009115.3

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