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CD163 ELISA Kit (Human)

CAT: 0247-OKEH02675-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKEH02675-96WSize:96 Well
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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CAS Number
7732-18-5
Gene Name
CD163 molecule
Gene Aliases
Hemoglobin scavenger receptor; M130; macrophage-associated antigen; MM130; SCARI1; scavenger receptor cysteine-rich type 1 protein M130.
Gene ID
9332
Accession Number
NP_004235.4
Reactivity
Homo sapiens, Human
Target
Scavenger receptor cysteine-rich type 1 protein M130
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology CD163 ELISA Kit (Human) (OKEH02675) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for CD163 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. After washing, a biotinylated detector antibody specific for CD163 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample CD163 captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
Detection Range
0.312-20ng/mL
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 106%
Sensitivity
0.1 ng/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
125 kDa
Specificity
Natural and recombinant Human Scavenger receptor cysteine-rich type 1 protein M130
Protein Length
1156
NCBI Gene Symbol
CD163
Protein Name
Q86VB7
Gene Name URL
CD163
Nucleotide Accession Number
NM_004244.5

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