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APP ELISA Kit (Human)

CAT: 0247-OKEH02647-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKEH02647-96WSize:96 Well
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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CAS Number
7732-18-5
Gene Name
Amyloid beta precursor protein
Gene Aliases
AAA; ABETA; ABPP; AD1; alpha-sAPP; alzheimer disease amyloid protein; amyloid beta (A4) precursor protein; amyloid beta A4 protein; amyloid precursor protein; amyloid-beta precursor protein; APPI; beta-amyloid peptide; beta-amyloid peptide (1-40) ; beta-amyloid peptide (1-42) ; beta-amyloid precursor protein; cerebral vascular amyloid peptide; CTFgamma; CVAP; peptidase nexin-II; PN2; PN-II; preA4; protease nexin-II; testicular tissue protein Li 2.
Gene ID
351
Accession Number
NP_000475.1
Reactivity
Homo sapiens, Human
Target
Amyloid-beta A4 protein
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology APP ELISA Kit (Human) (OKEH02647) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for APP has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. After washing, a biotinylated detector antibody specific for APP is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample APP captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
Detection Range
15.6-1000pg/mL
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 117%
Sensitivity
10 pg/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
87 kDa
Specificity
Natural and recombinant Human Amyloid beta A4 protein
Protein Length
770
NCBI Gene Symbol
APP
Protein Name
P05067
Gene Name URL
APP
Nucleotide Accession Number
NM_000484.3

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