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APP ELISA Kit (Rat)

CAT: 0247-OKCA00945-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKCA00945-96WSize:96 Well
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Reproducibility
Inter-Assay CV:
CAS Number
7732-18-5
Gene Name
Amyloid beta A4 protein
Accession Number
NP_001182598.1
Reactivity
Rat
Target
Functional neuronal receptor which couples to intracellular signaling pathway through the GTP-binding protein G (O) .
Type
ELISA Kit
Assay Principle
Aviva Systems Biology APP ELISA Kit (Rat) (OKCA00945) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. A antibody specific for APP has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) . Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for APP is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample APP captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates
Detection Range
15.6 ng/mL-1,000 ng/mL
Recovery
Matrix Recovery range (%) Average (%) Serum (n=5) 93 87-97 EDTA Plasma (n=4) 95 90-101 Matrix Recovery range (%) Average (%) Matrix Recovery range (%) Average (%) Serum (n=5) 93 87-97 Serum (n=5) 93 87-97 EDTA Plasma (n=4) 95 90-101 EDTA Plasma (n=4) 95 90-101
Sensitivity
3.9 ng/mL
Reconstitution
Store as indicated in product manual.
NCBI Gene Symbol
APP
Protein Name
Amyloid beta A4 protein
Gene Name URL
APP
Nucleotide Accession Number
NM_001195669.1

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