Products for Research Use Only
RegisterLogin

Nitrotyrosine ELISA Kit

CAT: 0247-OKEH02600-96WSize: 96 WellDry Ice: NoHazardous: No
Product image 1
1 / 1
CAT#:0247-OKEH02600-96WSize:96 Well
Selected
24/48H Stock Items & 2 to 6 Weeks non Stock Items.
Quick Request Actions
CAS Number
7732-18-5
Gene Aliases
NT, Nitrotyrosine, NT, 3-Nitrotyrosine, 3-Nitro-L-tyrosine, 3NT
Reactivity
All Species
Target
Nitrotyrosine is a product of tyrosine nitration mediated by reactive nitrogen species such as peroxynitrite anion and nitrogen dioxide. It is detected in large number of pathological conditions and is considered a marker of NO-dependent, reactive nitrogen species-induced nitrative stress. Nitrotyrosine is found in numerous disease-affected tissues, such as the cornea in keratoconus. Peroxynitrite and/or nitrative stress may participate in the pathogenesis of diabetes.
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Competitive
Assay Principle
Aviva Systems Biology Nitrotyrosine ELISA Kit (OKEH02600) is based on a competitive enzyme immunoassay technique. The microtiter well-plate in this kit has been pre-coated with an anti-Nitrotyrosine antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated Nitrotyrosine and incubated. The Nitrotyrosine found in the sample or standards competes with the biotinylated Nitrotyrosine for limited binding sites on the immobilized anti-Nitrotyrosine antibody. Excess unbound biotinylated Nitrotyrosine and sample or standard Nitrotyrosine is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated Nitrotyrosine captured in the well and inversely proportional to the amount of Nitrotyrosine which was contained in the sample or standard.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, plasma, tissue homogenates, cell culture supernatants and other biological fluids
Detection Range
15.6-1000pg/mL
Recovery
Mean recovery when spiking into Serum and Plasma = 81%
Sensitivity
4.9 pg/mL
Reconstitution
Store as indicated in product manual.
Specificity
Natural and recombinant General Nitrotyrosine
Protein Name
Nitrotyrosine

Chemical Information

Water

Water is an oxygen hydride consisting of an oxygen atom that is covalently bonded to two hydrogen atoms. It has a role as an amphiprotic solvent, a mouse metabolite, a Saccharomyces cerevisiae metabolite, a member of greenhouse gas, an Escherichia coli metabolite and a human metabolite. It is an oxygen hydride, a mononuclear parent hydride and an inorganic hydroxy compound. It is a conjugate base of an oxonium. It is a conjugate acid of a hydroxide.

CAS Number7732-18-5
PubChem CID962
IUPAC Nameoxidane
Molecular FormulaH2O
Molecular Weight18.015
XLogP-0.5
Topological Polar Surface Area1
Hydrogen Bond Donor Count1
Hydrogen Bond Acceptor Count1
Rotatable Bond Count0
Heavy Atom Count1
Formal Charge0
Complexity0
SMILES
O
InChI
InChI=1S/H2O/h1H2
InChIKey
XLYOFNOQVPJJNP-UHFFFAOYSA-N
Chemical Structure
2D Structure
2D structure of Water
CAS: 7732-18-5
CID: 962
Formula: H2O
MW: 18.015
Interactive 3D Structure
Loading 3D structure...
View on PubChemRaw 3D data
Computed Properties
PropertyValue
Molecular Weight18.015
XLogP3-0.5
Hydrogen Bond Donor Count1
Hydrogen Bond Acceptor Count1
Rotatable Bond Count0
Exact Mass18.010564683
Monoisotopic Mass18.010564683
Topological Polar Surface Area1 Ų
Heavy Atom Count1
Formal Charge0
Complexity0
Isotope Atom Count0
Defined Atom Stereocenter Count0
Undefined Atom Stereocenter Count0
Defined Bond Stereocenter Count0
Undefined Bond Stereocenter Count0
Covalently-Bonded Unit Count1
Compound Is CanonicalizedYes

Related Products

CatalogName
OAEE00505-100UGNitrotyrosine antibody
OAEG00227-100UGNitrotyrosine Antibody
OASA03588-100UGNITROTYROSINE Antibody
OASE00079-100UGNitrotyrosine Antibody
OASE00078-25UGOASE00078-25UG - Nitrotyrosine Antibody
OKCD06003-96WKIT ELISA Kit (Human)
OKEH07314-96WKIT ELISA Kit (Bovine)
OKEH07317-96WKIT ELISA Kit (Pig)
OKEH07316-96WKIT ELISA Kit (Dog)
OKEH00203-96WKIT ELISA Kit (Human)

Alternative Products

CatalogName
ECP7909-1Nitrotyrosine ELISA Kit
E1863GeELISA Kit For Nitrotyrosine
HK501-02Nitrotyrosine, ELISA kit
MBS721956-01Mouse Nitrotyrosine ELISA Kit
MBS762700-013-Nitrotyrosine ELISA Kit
MBS736339-01Monkey Nitrotyrosine ELISA Kit
MBS739798-01Chicken Nitrotyrosine ELISA Kit
SKT-126Nitrotyrosine ELISA Assay Kit
E100696General Nitrotyrosine ELISA Kit
KBH13922GENLISA Human Nitrotyrosine ELISA

Popular Products