S-Adenosyl methionine ELISA Kit

• Gene Aliases:

  SAM, S-Adenosyl methionine, SAM

• Reactivity:

  All Species

• Target:

  S-Adenosyl methionine (ademetionine, AdoMet, SAM, SAMe, SAM-e) is a common cosubstrate involved in methyl group transfers. SAM was first discovered in Italy by G. L. Cantoni in 1952. It is made from adenosine triphosphate (ATP) and methionine by methionine adenosyltransferase (EC 2.5.1.6) . Transmethylation, transsulfuration, and aminopropylation are the metabolic pathways that use SAM. Although these anabolic reactions occur throughout the body, most SAM is produced and consumed in the liver. The methyl group (CH3) attached to the methionine sulfur atom in SAM is chemically reactive. This allows donation of this group to an acceptor substrate in transmethylation reactions. More than 40 metabolic reactions involve the transfer of a methyl group from SAM to various substrates, such as nucleic acids, proteins, lipids and secondary metabolites. In bacteria, SAM is bound by the SAM riboswitch, which regulates genes involved in methionine or cysteine biosynthesis.

• Type:

  ELISA Kit

• Applications:

  Enzyme-linked Immunosorbent assay-Competitive

• Assay Principle:

  Aviva Systems Biology S-Adenosyl methionine ELISA Kit (OKEH02593) is based on a competitive enzyme immunoassay technique. The microtiter well-plate in this kit has been pre-coated with an anti-S-Adenosyl methionine antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated S-Adenosyl methionine and incubated. The S-Adenosyl methionine found in the sample or standards competes with the biotinylated S-Adenosyl methionine for limited binding sites on the immobilized anti-S-Adenosyl methionine antibody. Excess unbound biotinylated S-Adenosyl methionine and sample or standard S-Adenosyl methionine is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated S-Adenosyl methionine captured in the well and inversely proportional to the amount of S-Adenosyl methionine which was contained in the sample or standard.

• Assay Protocol:

  Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

• Sample Type:

  Serum, plasma, tissue homogenates, cell culture supernatants and other biological fluids

• Detection Range:

  1.56-100nmol/L

• Sensitivity:

  0.38nmol/L

• Reconstitution:

  Store as indicated in product manual.

• Specificity:

  Natural and recombinant General S-Adenosyl methionine

• Protein Name:

  S-Adenosyl methionine

• CAS Number:

  7732-18-5