HMBS ELISA Kit (Human)

CAT: 0247-OKEH02521-96WSize: 96 TestsDry Ice: NoHazardous: No
CAT#:0247-OKEH02521-96WSize:96 Tests
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CAS Number
7732-18-5
Gene Name
Hydroxymethylbilane synthase
Gene Aliases
Hydroxymethylbilane synthase; PBGD; PBG-D; PORC; porphobilinogen deaminase; porphyria, acute; Chester type; pre-uroporphyrinogen synthase; UPS; uroporphyrinogen I synthase; uroporphyrinogen I synthetase.
Gene ID
3145
Accession Number
NP_000181.2
Reactivity
Homo sapiens, Human
Target
Porphobilinogen deaminase
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology HMBS ELISA Kit (Human) (OKEH02521) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with an anti-Human HMBS antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated HMBS and incubated. The HMBS found in the sample or standards competes with the biotinylated HMBS for limited binding sites on the immobilized anti-Human HMBS antibody. Excess unbound biotinylated HMBS and sample or standard HMBS is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated HMBS captured in the well and inversely proportional to the amount of HMBS which was contained in the sample or standard.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
Detection Range
0.625-40ng/ml
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 94%
Sensitivity
0.327 ng/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
39 kDa
Specificity
Natural and recombinant Human Porphobilinogen deaminase
Protein Length
361
NCBI Gene Symbol
HMBS
Protein Name
P08397
Gene Name URL
HMBS
Nucleotide Accession Number
NM_000190.3

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