EPHB4 ELISA Kit (Mouse)

CAT: 0247-OKEH02461-96WSize: 96 TestsDry Ice: NoHazardous: No
CAT#:0247-OKEH02461-96WSize:96 Tests
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CAS Number
7732-18-5
Gene Name
Eph receptor B4
Gene Aliases
AI042935; b2b2412C; b2b2412Clo; developmental kinase 2; ephrin type-B receptor 4; hepatoma transmembrane kinase; Htk; mDK-2; MDK2; Myk1; Tyro; Tyro11; tyrosine kinase MYK-1.
Gene ID
13846
Accession Number
NP_034274.4
Reactivity
Mouse, Mus musculus
Target
Ephrin type-B receptor 4
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology EPHB4 ELISA Kit (Mouse) (OKEH02461) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for EPHB4 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for EPHB4 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample EPHB4 captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
Detection Range
31.2-2000pg/mL
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 81%
Sensitivity
15.62 pg/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
109 kDa
Specificity
Natural and recombinant Mouse Ephrin type-B receptor 4
Protein Length
987
NCBI Gene Symbol
Ephb4
Protein Name
P54761
Gene Name URL
Ephb4
Nucleotide Accession Number
NM_010144.6

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