Products for Research Use Only
RegisterLogin

H2AFX ELISA Kit (Mouse)

CAT: 0247-OKEH02433-96WSize: 96 TestsDry Ice: NoHazardous: No
Product image 1
1 / 1
CAT#:0247-OKEH02433-96WSize:96 Tests
Selected
24/48H Stock Items & 2 to 6 Weeks non Stock Items.
Quick Request Actions
CAS Number
7732-18-5
Gene Name
H2A.X variant histone
Gene Aliases
AW228881; gammaH; gammaH2ax; H2A histone family, member X; H2A.; H2A.X; H2a/x; H2af; H2afx; Hist5-; Hist5-2ax; histone 5 protein 2ax; histone H2A.x; histone H2AX.
Gene ID
15270
Accession Number
NP_034566.1
Reactivity
Mouse, Mus musculus
Target
Histone H2AX
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology H2AFX ELISA Kit (Mouse) (OKEH02433) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for H2AFX has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for H2AFX is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample H2AFX captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
Detection Range
31.2-2000pg/mL
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 97%
Sensitivity
15.61 pg/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
15 kDa
Specificity
Natural and recombinant Mouse Histone H2AX
Protein Length
143
NCBI Gene Symbol
H2ax
Protein Name
P27661
Gene Name URL
H2ax
Nucleotide Accession Number
NM_010436.2

Related Products

CatalogName
OKCD02089-96WH2AFX ELISA Kit (Human)
OKCD02089H2AFX ELISA Kit (Human) (OKCD02089)
OACA04317-100UGH2AFX Antibody
OACA07428-100ULH2AFX Antibody
OACA07428-50ULH2AFX Antibody
OAAJ02988-100ULH2AFX Antibody
OACA04317-50UGH2AFX Antibody
OASG03382-100ULH2AFX Antibody
OCOA00690-20UGH2AFX Protein Lysate
OPCD03780-10UGH2AFX Recombinant Protein

Alternative Products

CatalogName
E055161Mouse H2AFX ELISA Kit
abx254988Mouse Histone H2AX (H2AFX) ELISA Kit
E0645MoMouse H2afx/Histone H2AX ELISA Kit
RK13210Mouse Histone H2A.x (H2AFX) ELISA Kit
KLM5685GENLISA™ Mouse Histone H2A.x (H2afx) ELISA
E0597Histone H2A.x (H2AFX) Mouse ELISA Kit
ELI-39065mMouse Histone H2A.x, H2afx ELISA KIT
EM0637Mouse H2afx (Histone H2A.x) ELISA Kit
EK250288Mouse H2AFX (H2A Histone Family, Member X) ELISA Kit
EK732404Mouse H2A Histone Family, Member X (H2AFX) Elisa Kit

Popular Products