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GUCY2C ELISA Kit (Human)

CAT: 0247-OKEH02382-96WSize: 96 TestsDry Ice: NoHazardous: No
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CAT#:0247-OKEH02382-96WSize:96 Tests
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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CAS Number
7732-18-5
Gene Name
Guanylate cyclase 2C
Gene Aliases
DIAR6; GC-C; guanylyl cyclase C; GUC2C; heat-stable enterotoxin receptor; intestinal guanylate cyclase; MECIL; MUCIL; STA receptor; STAR.
Gene ID
2984
Accession Number
NP_004954.2
Reactivity
Homo sapiens, Human
Target
Heat-stable enterotoxin receptor
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Competitive
Assay Principle
Aviva Systems Biology GUCY2C ELISA Kit (Human) (OKEH02382) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for GUCY2C has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for GUCY2C is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample GUCY2C captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
Detection Range
0.156-10ng/mL
Sensitivity
0.078 ng/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
123 kDa
Specificity
Natural and recombinant Human Heat-stable enterotoxin receptor
Protein Length
1073
NCBI Gene Symbol
GUCY2C
Protein Name
P25092
Gene Name URL
GUCY2C
Nucleotide Accession Number
NM_004963.3

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