PHF1 ELISA Kit (Human)

CAT: 0247-OKEH02297-96WSize: 96 WellDry Ice: NoHazardous: No
CAT#:0247-OKEH02297-96WSize:96 Well
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CAS Number
7732-18-5
Gene Name
PHD finger protein 1
Gene Aliases
HPCl1; hPHF1; MTF2L2; PCL1; PHD finger protein 1; PHF2; polycomb-like 1; polycomb-like protein 1; TDRD19C; testicular tissue protein Li 140; tudor domain containing 19C.
Gene ID
5252
Accession Number
NP_002627.1
Reactivity
Homo sapiens, Human
Target
PHD finger protein 1
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology PHF1 ELISA Kit (Human) (OKEH02297) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for PHF1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for PHF1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample PHF1 captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
Detection Range
0.312-20ng/mL
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 111%
Sensitivity
0.21 ng/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
62 kDa
Specificity
Natural and recombinant Human PHD finger protein 1
Protein Length
567
NCBI Gene Symbol
PHF1
Protein Name
O43189
Gene Name URL
PHF1
Nucleotide Accession Number
NM_002636.4

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