MAT2A ELISA Kit (Human)

7732-18-5

Methionine adenosyltransferase 2A

AdoMet synthase 2; adoMet synthetase 2; MAT 2; MATA2; MATII; MAT-II; methionine adenosyltransferase 2; Methionine adenosyltransferase II; methionine adenosyltransferase II, alpha; S-adenosylmethionine synthase isoform type-2; SAMS2; testicular tissue protein Li 121.

4144

NP_005902.1

Homo sapiens, Human

S-adenosylmethionine synthase isoform type-2

ELISA Kit

Enzyme-linked Immunosorbent assay-Sandwich

Aviva Systems Biology MAT2A ELISA Kit (Human) (OKEH02254) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for MAT2A has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for MAT2A is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample MAT2A captured in well.

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids

0.156-10ng/mL

Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 107%

0.15 ng/mL

Store as indicated in product manual.

44 kDa

Natural and recombinant Human S-adenosylmethionine synthase isoform type-2

395

MAT2A

P31153

MAT2A