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RRM2 ELISA Kit (Rat)

CAT: 0247-OKEH02214-96WSize: 96 TestsDry Ice: NoHazardous: No
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CAT#:0247-OKEH02214-96WSize:96 Tests
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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CAS Number
7732-18-5
Gene Name
Ribonucleotide reductase regulatory subunit M2
Gene Aliases
Ribonucleoside-diphosphate reductase subunit M2; Ribonucleotide reductase 2; ribonucleotide reductase M2 polypeptide; ribonucleotide reductase small chain; ribonucleotide reductase small subunit.
Gene ID
362720
Accession Number
NP_001020911.1
Reactivity
Rat, Rattus norvegicus
Target
Ribonucleoside-diphosphate reductase subunit M2
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Competitive
Assay Principle
Aviva Systems Biology RRM2 ELISA Kit (Rat) (OKEH02214) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with an anti-Rat RRM2 antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated RRM2 and incubated. The RRM2 found in the sample or standards competes with the biotinylated RRM2 for limited binding sites on the immobilized anti-Rat RRM2 antibody. Excess unbound biotinylated RRM2 and sample or standard RRM2 is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated RRM2 captured in the well and inversely proportional to the amount of RRM2 which was contained in the sample or standard.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
Detection Range
0.312-20ng/mL
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 96%
Sensitivity
0.156 ng/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
45 kDa
Specificity
Natural and recombinant Rat Ribonucleoside-diphosphate reductase subunit M2
Protein Length
390
NCBI Gene Symbol
Rrm2
Protein Name
Q4KLN6
Gene Name URL
Rrm2
Nucleotide Accession Number
NM_001025740.1

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