TMPRSS4 ELISA Kit (Mouse)

7732-18-5

Transmembrane protease, serine 4

CAP2; channel-activating protease 2; channel-activating protease 4; mCAP2; membrane-type serine protease 2; transmembrane protease serine 4.

214523

NP_663378.1

Mouse, Mus musculus

Transmembrane protease serine 4

ELISA Kit

Enzyme-linked Immunosorbent assay-Sandwich

Aviva Systems Biology TMPRSS4 ELISA Kit (Mouse) (OKEH02206) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with an anti-Mouse TMPRSS4 antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated TMPRSS4 and incubated. The TMPRSS4 found in the sample or standards competes with the biotinylated TMPRSS4 for limited binding sites on the immobilized anti-Mouse TMPRSS4 antibody. Excess unbound biotinylated TMPRSS4 and sample or standard TMPRSS4 is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated TMPRSS4 captured in the well and inversely proportional to the amount of TMPRSS4 which was contained in the sample or standard.

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids

1.56-100ng/mL

Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 102%

0.786 ng/mL

Store as indicated in product manual.

47 kDa

Natural and recombinant Mouse Transmembrane protease serine 4

435

Tmprss4

Q8VCA5

Tmprss4