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NEFL ELISA Kit (Human)

CAT: 0247-OKEH02111-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKEH02111-96WSize:96 Well
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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CAS Number
7732-18-5
Gene Name
Neurofilament light chain
Gene Aliases
68 kDa neurofilament protein; CMT1F; CMT2E; CMTDIG; light molecular weight neurofilament protein; neurofilament light polypeptide; neurofilament protein, light chain; neurofilament subunit NF-L; neurofilament triplet L protein; neurofilament, light polypeptide 68kDa; NF68; NFL; NF-L; PPP1R110; protein phosphatase 1, regulatory subunit 110.
Gene ID
4747
Accession Number
NP_006149.2
Reactivity
Homo sapiens, Human
Target
Neurofilament light polypeptide
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology NEFL ELISA Kit (Human) (OKEH02111) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for NEFL has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for NEFL is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample NEFL captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
Detection Range
31.2-2000pg/mL
Sensitivity
10 pg/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
62 kDa
Notes
Formerly GWB-9VALPK
Specificity
Natural and recombinant Human Neurofilament light polypeptide
Protein Length
543
NCBI Gene Symbol
NEFL
Protein Name
P07196
Gene Name URL
NEFL
Nucleotide Accession Number
NM_006158.4

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