MAP2K5 ELISA Kit (Human)

CAT: 0247-OKEH02078-96WSize: 96 WellDry Ice: NoHazardous: No
CAT#:0247-OKEH02078-96WSize:96 Well
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CAS Number
7732-18-5
Gene Name
Mitogen-activated protein kinase kinase 5
Gene Aliases
Dual specificity mitogen-activated protein kinase kinase 5; HsT17454; MAP kinase kinase 5; MAP kinase kinase MEK5b; MAPK/ERK kinase 5; MAPKK 5; MAPKK5; MEK 5; MEK5; PRKMK5.
Gene ID
5607
Accession Number
NP_001193733.1
Reactivity
Homo sapiens, Human
Target
Dual specificity mitogen-activated protein kinase kinase 5
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology MAP2K5 ELISA Kit (Human) (OKEH02078) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for MAP2K5 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for MAP2K5 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample MAP2K5 captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Urine, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
Detection Range
0.312-20ng/mL
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 101%
Sensitivity
0.11 ng/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
50 kDa
Specificity
Natural and recombinant Human Dual specificity mitogen-activated protein kinase kinase 5
Protein Length
448
NCBI Gene Symbol
MAP2K5
Protein Name
Q13163
Gene Name URL
MAP2K5
Nucleotide Accession Number
NM_001206804.1

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