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PRKAA2 ELISA Kit (Human)

CAT: 0247-OKEH02064-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKEH02064-96WSize:96 Well
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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CAS Number
7732-18-5
Gene Name
Protein kinase AMP-activated catalytic subunit alpha 2
Gene Aliases
5'-AMP-activated protein kinase catalytic subunit alpha-2;5'-AMP-activated protein kinase, catalytic alpha-2 chain; ACACA kinase; acetyl-CoA carboxylase kinase; AMP-activated protein kinase alpha-2 subunit variant 2; AMP-activated protein kinase alpha-2 subunit variant 3; AMPK; AMPK alpha 2; AMPK subunit alpha-2; AMPK2; AMPKa2; AMPK-alpha-2 chain; HMGCR kinase; hydroxymethylglutaryl-CoA reductase kinase; PRKAA; protein kinase, AMP-activated, alpha 2 catalytic subunit.
Gene ID
5563
Accession Number
NP_006243.2
Reactivity
Homo sapiens, Human
Target
5'-AMP-activated protein kinase catalytic subunit alpha-2
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology PRKAA2 ELISA Kit (Human) (OKEH02064) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for PRKAA2 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for PRKAA2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample PRKAA2 captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
Detection Range
0.156-10ng/mL
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 100%
Sensitivity
0.078 ng/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
62 kDa
Specificity
Natural and recombinant Human 5'-AMP-activated protein kinase catalytic subunit alpha-2
Protein Length
552
NCBI Gene Symbol
PRKAA2
Protein Name
P54646
Gene Name URL
PRKAA2
Nucleotide Accession Number
NM_006252.3

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