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EAPA2 ELISA Kit (Mouse)

CAT: 0247-OKEH01862-96WSize: 96 TestsDry Ice: NoHazardous: No
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CAT#:0247-OKEH01862-96WSize:96 Tests
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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CAS Number
7732-18-5
Gene Name
TRPM8 channel-associated factor 3
Gene Aliases
Ea; Eapa2; experimental autoimmune prostatitis antigen 2; Fam115; Fam115e; family with sequence similarity 115, member E; protein FAM115E; TRPM8 channel-associated factor 3.
Gene ID
403088
Accession Number
NP_981933.1
Reactivity
Mouse, Mus musculus
Target
TRPM8 channel-associated factor 3
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology EAPA2 ELISA Kit (Mouse) (OKEH01862) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for EAPA2 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for EAPA2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample EAPA2 captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
Detection Range
0.156-10ng/mL
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 101%
Sensitivity
0.078 ng/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
102 kDa
Specificity
Natural and recombinant Mouse Experimental autoimmune prostatitis antigen 2
Protein Length
914
NCBI Gene Symbol
Tcaf3
Protein Name
Q6QR59
Gene Name URL
Tcaf3
Nucleotide Accession Number
NM_203396.1

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