Products for Research Use Only
RegisterLogin

PYCARD ELISA Kit (Human)

CAT: 0247-OKEH01695-96WSize: 96 WellDry Ice: NoHazardous: No
Product image 1
1 / 1
CAT#:0247-OKEH01695-96WSize:96 Well
Selected
24/48H Stock Items & 2 to 6 Weeks non Stock Items.
Quick Request Actions
CAS Number
7732-18-5
Gene Name
PYD and CARD domain containing
Gene Aliases
Apoptosis-associated speck-like protein containing a CARD; ASC; CARD5; caspase recruitment domain-containing protein 5; PYD and CARD domain-containing protein; target of methylation-induced silencing 1; TMS; TMS1; TMS-1.
Gene ID
29108
Accession Number
NP_037390.2
Reactivity
Homo sapiens, Human
Target
Apoptosis-associated speck-like protein containing a CARD
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology PYCARD ELISA Kit (Human) (OKEH01695) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for PYCARD has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for PYCARD is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample PYCARD captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids
Detection Range
0.156-10ng/mL
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 90%
Sensitivity
0.078 ng/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
22 kDa
Specificity
Natural and recombinant Human Apoptosis-associated speck-like protein containing a CARD
Protein Length
195
NCBI Gene Symbol
PYCARD
Protein Name
Q9ULZ3
Gene Name URL
PYCARD
Nucleotide Accession Number
NM_013258.4

Related Products

CatalogName
OKEH05135-96WPYCARD ELISA Kit (Mouse)
OKEH05135PYCARD ELISA Kit (Mouse) (OKEH05135)
OAAN00256-100ULPYCARD Antibody
OAAN00256-50ULPYCARD Antibody
OAAN00256-200ULPYCARD Antibody
OAAF02748-100UGPYCARD Antibody
OCOA06105-20UGPYCARD Protein Lysate
OCOA02030-20UGPYCARD Protein Lysate
AAP37320-100UGPYCARD Peptide (AAP37320)
OAEB03148-100UGPYCARD Antibody - middle region

Alternative Products

CatalogName
ELI-34333hHuman PYCARD ELISA KIT
GTR18830975-01Human PYCARD ELISA Kit (Ready to Use)
abx520248Human PYD And CARD Domain Containing (PYCARD) ELISA Kit
GTR18868734-01Human PYD And CARD Domain Containing Protein (PYCARD) ELISA Kit
MBS8808122-01Human PYCARD (PYD And CARD Domain Containing Protein) ELISA Kit
KBH15395GENLISA Human PYD And CARD Domain Containing (PYCARD/TMS1) ELISA
201-12-5368Human PYD and CARD domain containing (PYCARD/TMS1) ELISA Kit
JL15256-01Human PYD And CARD Domain Containing Protein (PYCARD) ELISA Kit
abx508190Human Putative uncharacterized protein C16Orf98 (PYCARD-AS1) ELISA Kit
MBS8203830-01PYCARD siRNA (Human)

Popular Products