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SLC6A4 ELISA Kit (Mouse)

CAT: 0247-OKEH01624-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKEH01624-96WSize:96 Well
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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CAS Number
7732-18-5
Gene Name
Solute carrier family 6 (neurotransmitter transporter, serotonin), member 4
Gene Aliases
5HT transporter;5HTT;5-HTT;5-hydroxytryptamine (serotonin) transporter; AI323329; Htt; Se; Sert; sodium-dependent serotonin transporter; Sodium-dependent serotonin transporter (5HT transporter) (5HTT) ; solute carrier family 6 member 4.
Gene ID
15567
Accession Number
NP_034614.2
Reactivity
Mouse, Mus musculus
Target
Sodium-dependent serotonin transporter
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology SLC6A4 ELISA Kit (Mouse) (OKEH01624) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for SLC6A4 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for SLC6A4 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample SLC6A4 captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids
Detection Range
15.6-1000pg/mL
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 104%
Sensitivity
8.02 pg/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
70 kDa
Specificity
Natural and recombinant Mouse Sodium-dependent serotonin transporter
Protein Length
630
NCBI Gene Symbol
Slc6a4
Protein Name
Q60857
Gene Name URL
Slc6a4
Nucleotide Accession Number
NM_010484.2

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