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PRDX1 ELISA Kit (Mouse)

CAT: 0247-OKEH01573-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKEH01573-96WSize:96 Well
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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CAS Number
7732-18-5
Gene Name
Peroxiredoxin 1
Gene Aliases
Macrophage 23 Kd stress protein; macrophage 23 kDa stress protein; macrophage 23kDa stress protein; macrophase stress protein 22kDa; macrophase stress protein 23 kd; MSP; MSP23; NkefA; OSF; OSF3; OSF-3; osteoblast-specific factor 3; P; PAG; Paga; peroxiredoxin-1; pr; PrdxI; proliferation-associated gene A; prx1; PrxI; Tdpx; Tdpx2; TDX2; thioredoxin peroxidase 2; thioredoxin-dependent peroxide reductase 2; thioredoxin-dependent peroxiredoxin 1; TPxA; Trx dependent peroxide reductase 2.
Gene ID
18477
Accession Number
NP_035164.1
Reactivity
Mouse, Mus musculus
Target
Peroxiredoxin-1
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology PRDX1 ELISA Kit (Mouse) (OKEH01573) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for PRDX1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for PRDX1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample PRDX1 captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids
Detection Range
0.156-10ng/mL
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 89%
Sensitivity
0.078 ng/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
22 kDa
Specificity
Natural and recombinant Mouse Peroxiredoxin-1
Protein Length
199
NCBI Gene Symbol
Prdx1
Protein Name
P35700
Gene Name URL
Prdx1
Nucleotide Accession Number
NM_011034.4

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