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CASP8 ELISA Kit (Human)

CAT: 0247-OKEH01430-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKEH01430-96WSize:96 Well
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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CAS Number
7732-18-5
Gene Name
Caspase 8
Gene Aliases
ALPS2B; apoptotic cysteine protease; apoptotic protease Mch-5; CAP4; Casp-8; caspase 8, apoptosis-related cysteine peptidase; caspase 8, apoptosis-related cysteine protease; caspase-8; FADD-homologous ICE/CED-3-like protease; FADD-like ICE; FLICE; ICE-like apoptotic protease 5; MACH; MACH-alpha-1/2/3 protein; MACH-beta-1/2/3/4 protein; MCH5; MORT1-associated ced-3 homolog.
Gene ID
841
Accession Number
NP_001073593.1
Reactivity
Homo sapiens, Human
Target
Caspase-8
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology CASP8 ELISA Kit (Human) (OKEH01430) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for CASP8 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for CASP8 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample CASP8 captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids
Detection Range
0.156-10ng/mL
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 83%
Sensitivity
0.06 ng/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
55 kDa
Specificity
Natural and recombinant Human Caspase-8
Protein Length
479
NCBI Gene Symbol
CASP8
Protein Name
Q14790
Gene Name URL
CASP8
Nucleotide Accession Number
NM_001080124.1

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