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MAPKAPK3 ELISA Kit (Rat)

CAT: 0247-OKEH01178-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKEH01178-96WSize:96 Well
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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CAS Number
7732-18-5
Gene Name
MAPK activated protein kinase 3
Gene Aliases
MAP kinase-activated protein kinase 3; MAPKAP kinase 3; MAPKAPK-3; MAPKAP-K3; mitogen-activated protein kinase-activated protein kinase 3; MK-3.
Gene ID
315994
Accession Number
NP_001012127.1
Reactivity
Rat, Rattus norvegicus
Target
MAP kinase-activated protein kinase 3
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology MAPKAPK3 ELISA Kit (Rat) (OKEH01178) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for MAPKAPK3 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for MAPKAPK3 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample MAPKAPK3 captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids
Detection Range
0.312-20ng/mL
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 82%
Sensitivity
0.186 ng/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
43 kDa
Specificity
Natural and recombinant Rat MAP kinase-activated protein kinase 3
Protein Length
384
NCBI Gene Symbol
Mapkapk3
Protein Name
Q66H84
Gene Name URL
Mapkapk3
Nucleotide Accession Number
NM_001012127.1

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