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DID ELISA Kit (Mouse)

CAT: 0247-OKEH00588-96WSize: 96 TestsDry Ice: NoHazardous: No
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CAT#:0247-OKEH00588-96WSize:96 Tests
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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CAS Number
7732-18-5
Gene Name
Dihydrolipoamide dehydrogenase
Gene Aliases
Branched chain alpha-keto acid dehydrogenase complex subunit E3; Dihydrolipoamide dehydrogenase; dihydrolipoyl dehydrogenase, mitochondrial.
Gene ID
13382
Reactivity
Mouse, Mus musculus
Target
Dihydrolipoyl dehydrogenase, mitochondrial
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology ELISA Kit (Mouse) (OKEH00588) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with an anti-Mouse KNG1 antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated KNG1 and incubated. The KNG1 found in the sample or standards competes with the biotinylated KNG1 for limited binding sites on the immobilized anti-Mouse KNG1 antibody. Excess unbound biotinylated KNG1 and sample or standard KNG1 is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated KNG1 captured in the well and inversely proportional to the amount of KNG1 which was contained in the sample or standard.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
Detection Range
0.78-50ng/mL
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 100%
Sensitivity
0.46 ng/mL
Reconstitution
Store as indicated in product manual.
Specificity
Natural and recombinant Mouse Dihydrolipoyl dehydrogenase, mitochondrial
NCBI Gene Symbol
Dld
Protein Name
O08749
Gene Name URL
Dld

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