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TRIM22 ELISA Kit (Human)

CAT: 0247-OKEH00575-96WSize: 96 TestsDry Ice: NoHazardous: No
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CAT#:0247-OKEH00575-96WSize:96 Tests
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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CAS Number
7732-18-5
Gene Name
Tripartite motif containing 22
Gene Aliases
50 kDa-stimulated trans-acting factor; E3 ubiquitin-protein ligase TRIM22; GPSTAF50; RING finger protein 94; RING-type E3 ubiquitin transferase TRIM22; RNF94; staf-50; STAF50; stimulated trans-acting factor (50 kDa) ; tripartite binding motif 22; tripartite motif protein TRIM22; tripartite motif-containing protein 22.
Gene ID
10346
Accession Number
NP_001186502.1
Reactivity
Homo sapiens, Human
Target
E3 ubiquitin-protein ligase TRIM22
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology TRIM22 ELISA Kit (Human) (OKEH00575) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Human TRIM22 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. After washing, a biotinylated detector antibody specific for Human TRIM22 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Human TRIM22 captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
Detection Range
0.312-20ng/mL
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 83%
Sensitivity
0.11 ng/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
57 kDa
Specificity
Natural and recombinant Human E3 ubiquitin-protein ligase TRIM22
Protein Length
498
NCBI Gene Symbol
TRIM22
Protein Name
Q8IYM9
Gene Name URL
TRIM22
Nucleotide Accession Number
NM_001199573.1

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