REG1A ELISA Kit (Human)

CAT: 0247-OKEH00562-96WSize: 96 WellDry Ice: NoHazardous: No
CAT#:0247-OKEH00562-96WSize:96 Well
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CAS Number
7732-18-5
Gene Name
Regenerating family member 1 alpha
Gene Aliases
ICRF; islet cells regeneration factor; islet of langerhans regenerating protein; lithostathine-1-alpha; P19; Pancreatic stone protein; pancreatic stone protein, secretory; pancreatic thread protein; protein-X; PSP; PSPS; PSPS1; PTP; REG; REG-1-alpha; regenerating islet-derived protein 1-alpha; regenerating protein I alpha.
Gene ID
5967
Accession Number
NP_002900.2
Reactivity
Homo sapiens, Human
Target
Lithostathine-1-alpha
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology REG1A ELISA Kit (Human) (OKEH00562) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Human REG1A has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. After washing, a biotinylated detector antibody specific for Human REG1A is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Human REG1A captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
Detection Range
1.56-100ng/mL
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 96%
Sensitivity
0.39 ng/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
19 kDa
Specificity
Natural and recombinant Human Lithostathine-1-alpha
Protein Length
166
NCBI Gene Symbol
REG1A
Protein Name
P05451
Gene Name URL
REG1A
Nucleotide Accession Number
NM_002909.4

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