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NOS2 ELISA Kit (Rat)

CAT: 0247-OKEH00555-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKEH00555-96WSize:96 Well
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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CAS Number
7732-18-5
Gene Name
Nitric oxide synthase 2
Gene Aliases
Inducible nitric oxide synthase; inducible NO synthase; inducible NOS; iNos; nitric oxide synthase 2, inducible, macrophage; nitric oxide synthase, inducible; nitric oxide synthase, inducible-like; NOS type II; Nos2a; peptidyl-cysteine S-nitrosylase NOS2.
Gene ID
24599
Accession Number
NP_036743.3
Reactivity
Rat, Rattus norvegicus
Target
Nitric oxide synthase, inducible
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology NOS2 ELISA Kit (Rat) (OKEH00555) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with an anti-Rat NOS2 antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated NOS2 and incubated. The NOS2 found in the sample or standards competes with the biotinylated NOS2 for limited binding sites on the immobilized anti-Rat NOS2 antibody. Excess unbound biotinylated NOS2 and sample or standard NOS2 is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated NOS2 captured in the well and inversely proportional to the amount of NOS2 which was contained in the sample or standard.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
Detection Range
0.156-10ng/mL
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 102%
Sensitivity
0.78nmol/L
Reconstitution
Store as indicated in product manual.
Molecular Weight
131 kDa
Specificity
Natural and recombinant Rat Nitric oxide synthase, inducible
Protein Length
1147
NCBI Gene Symbol
Nos2
Protein Name
Q06518
Gene Name URL
Nos2
Nucleotide Accession Number
NM_012611.3

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