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FGF2 ELISA Kit (Human)

CAT: 0247-OKEH00519-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKEH00519-96WSize:96 Well
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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CAS Number
7732-18-5
Gene Name
Fibroblast growth factor 2
Gene Aliases
Basic fibroblast growth factor; basic fibroblast growth factor bFGF; BFGF; FGF-2; FGFB; fibroblast growth factor 2; fibroblast growth factor 2 (basic) ; HBGF-2; heparin-binding growth factor 2; prostatropin.
Gene ID
2247
Accession Number
NP_001997.5
Reactivity
Homo sapiens, Human
Target
Fibroblast growth factor 2
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology FGF2 ELISA Kit (Human) (OKEH00519) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Human FGF2 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. After washing, a biotinylated detector antibody specific for Human FGF2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Human FGF2 captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
Detection Range
1.56-100pg/mL
Recovery
<= 5.5%
Sensitivity
10 pg/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
31 kDa
Specificity
Natural and recombinant Human Fibroblast growth factor 2
Protein Length
288
NCBI Gene Symbol
FGF2
Protein Name
P09038
Gene Name URL
FGF2
Nucleotide Accession Number
NM_002006.4

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