CD55 ELISA Kit (Human)

CAT: 0247-OKEH00460-96WSize: 96 WellDry Ice: NoHazardous: No
CAT#:0247-OKEH00460-96WSize:96 Well
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CAS Number
7732-18-5
Gene Name
CD55 molecule (Cromer blood group)
Gene Aliases
CD55 antigen; CD55 molecule, decay accelerating factor for complement (Cromer blood group) ; CHAPLE; complement decay-accelerating factor; CR; CROM; Cromer blood group antigen; DAF; Rh blood group D antigen; TC.
Gene ID
1604
Accession Number
NP_000565.1
Reactivity
Homo sapiens, Human
Target
Complement decay-accelerating factor
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology CD55 ELISA Kit (Human) (OKEH00460) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Human CD55 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. After washing, a biotinylated detector antibody specific for Human CD55 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Human CD55 captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
Detection Range
31.2-2000pg/mL
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 101%
Sensitivity
10.3 pg/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
41 kDa
Specificity
Natural and recombinant Human Complement decay-accelerating factor
Protein Length
381
NCBI Gene Symbol
CD55
Protein Name
P08174
Gene Name URL
CD55
Nucleotide Accession Number
NM_000574.4

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