Cd55 ELISA Kit (Mouse)

• Reproducibility:

  Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 225 925 4690 308 1190 4853 Standard deviation 9.45 39.78 192.29 20.33 80.92 344.56 CV (%) 4.2 4.3 4.1 6.6 6.8 7.1 Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 225 925 4690 308 1190 4853 Mean (pg/ml) 225 925 4690 308 1190 4853 Standard deviation 9.45 39.78 192.29 20.33 80.92 344.56 Standard deviation 9.45 39.78 192.29 20.33 80.92 344.56 CV (%) 4.2 4.3 4.1 6.6 6.8 7.1 CV (%) 4.2 4.3 4.1 6.6 6.8 7.1

• Gene Name:

  CD55 molecule, decay accelerating factor for complement

• Gene Aliases:

  Complement decay-accelerating factor; GPI-anchored; DAF-GPI; CD55; Cd55

• Gene ID:

  13136

• Accession Number:

  NP_034146.2

• Reactivity:

  Mouse

• Immunogen:

  Expression system for standard: NS0; Immunogen sequence: D35-G362

• Target:

  Complement decay-accelerating factor, also known as CD55 or DAF, is a protein that, in humans, is encoded by the CD55 gene. It is mapped to 1 E4; 1 56.89 cM. This gene encodes a glycoprotein involved in the regulation of the complement cascade. Binding of the encoded protein to complement proteins accelerates their decay, thereby disrupting the cascade and preventing damage to host cells. Antigens present on this protein constitute the Cromer blood group system (CROM) . Alternative splicing results in multiple transcript variants. The predominant transcript variant encodes a membrane-bound protein, but alternatively spliced transcripts may produce soluble proteins.

• Type:

  ELISA Kit

• Applications:

  ELISA

• Assay Principle:

  Aviva's mouse CD55 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for CD55 has been precoated onto 96-well plates. Standards (Expression system for standard: NS0, Immunogen sequence: D35-G362) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CD55 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The absorbance of yellow is proportional to the human Fibronectin amount of sample captured in plate.

• Assay Protocol:

  Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

• Sample Type:

  Cell culture supernates, serum and plasma (heparin, EDTA)

• Detection Range:

  156 pg/ml - 10,000 pg/ml

• Sensitivity:

  <50 pg/ml

• Reconstitution:

  Store at 4C for 6 months, at -20C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

• Specificity:

  Natural and recombinant mouse CD55

• NCBI Gene Symbol:

  CD55

• Protein Name:

  Complement decay-accelerating factor, GPI-anchored

• Gene Name URL:

  CD55

• CAS Number:

  7732-18-5

• Nucleotide Accession Number:

  NM_010016.3