HSF1 ELISA Kit (Human)

CAT: 0247-OKEH00444-96WSize: 96 WellDry Ice: NoHazardous: No
CAT#:0247-OKEH00444-96WSize:96 Well
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CAS Number
7732-18-5
Gene Name
Heat shock transcription factor 1
Gene Aliases
Heat shock factor protein 1; Heat shock transcription factor 1; HSTF1.
Gene ID
3297
Accession Number
NP_005517.1
Reactivity
Homo sapiens, Human
Target
Heat shock factor protein 1
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology HSF1 ELISA Kit (Human) (OKEH00444) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Human HSF1 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. After washing, a biotinylated detector antibody specific for Human HSF1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Human HSF1 captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
Detection Range
0.312-20ng/mL
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 103%
Sensitivity
0.11 ng/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
57 kDa
Specificity
Natural and recombinant Human Heat shock factor protein 1
Protein Length
529
NCBI Gene Symbol
HSF1
Protein Name
Q00613
Gene Name URL
HSF1
Nucleotide Accession Number
NM_005526.2

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