Products for Research Use Only
RegisterLogin

HSF1 Antibody

CAT: 0247-OASE00323-100UGSize: 100 µgDry Ice: NoHazardous: No
Product image 1
1 / 1
CAT#:0247-OASE00323-100UGSize:100 µg
Selected
24/48H Stock Items & 2 to 6 Weeks non Stock Items.
Quick Request Actions
CAS Number
9007-83-4
Gene Name
Heat shock transcription factor 1
Gene Aliases
HSTF1, Heat shock factor protein 1, Heat shock transcription factor 1, HSF 1
Gene ID
15499
Accession Number
NP_032322.1
Reactivity
Human, Mouse, Rat, Bovine, Guinea Pig, Hamster, Monkey, Rabbit
Immunogen
Purified recombinant mouse HSF1 protein, epitope mapping to amino acids 425-439
Target
HSF1, or heat shock factor 1, belongs to a family of Heat Shock transcription factors that activate the transcription of genes encoding products required for protein folding, processing, targeting, degradation, and function (2) . The up-regulation of HSP (heat shock proteins) expression by stressors is achieved at the level of transcription through a heat shock element (HSE) and a transcription factor (HSF) (3, 4, 5) . Most HSFs have highly conserved amino acid sequences. On all HSFs there is a DNA binding domain at the N-terminus. Hydrophobic repeats located adjacent to this binding domain are essential for the formation of active trimers. Towards the C-terminal region another short hydrophobic repeat exists, and is thought to be necessary for suppression of trimerization (6) . There are two main heat shock factors, 1 and 2. Mouse HSF1 exists as two isoforms, however in higher eukaryotes HSF1 is found in a diffuse cytoplasmic and nuclear distribution in un-stressed cells. Once exposed to a multitude of stressors, it localizes to discrete nuclear granules within seconds. As it recovers from stress, HSF1 dissipates from these granules to a diffuse nuceloplasmic distribution. HSF2 on the other hand is similar to mouse HSF1, as it exists as two isoforms, the alpha form being more transcriptionally active than the smaller beta form (7, 8) . Various experiments have suggested that HFS2 may have roles in differentiation and development (9, 10, 11) .
Clonality
Monoclonal
Clone
4B4
Conjugation
Unconjugated
Type
Monoclonal Antibody
Applications
WB, IHC, ICC, IF, IP, ELISA, GS
Purification
Protein G Purified
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Concentration
1 mg/ml
Dilution
WB (1:1000), IHC (1:2000), ICC/IF (1:100) ; optimal dilutions for assays should be determined by the user.
Format
Liquid// PBS pH 7.4, 50% glycerol, 0.1% sodium azide
Buffer
PBS pH 7.4, 50% glycerol, 0.1% sodium azide
Reconstitution
Store at -20C. Shipping with Blue Ice or 4C.
Molecular Weight
Detects ~85kDa (unstressed cell lysates) and ~95kDa (heat shocked cell lysates) .
Fragment
IgG1
Specificity
Detects ~85kDa (unstressed cell lysates) and ~95kDa (heat shocked cell lysates) .
NCBI Gene Symbol
HSF1
Host or Source
Rat
Gene Name URL
HSF1

Related Products

CatalogName
OPPA01698-5UGHSF1 Protein
OAED00212-50UGAnti-HSF1 (10H8) Antibody
OPCD03993-200UGHSF1 Recombinant Protein
OPCD03993-10UGHSF1 Recombinant Protein
AAP36895-100UGHSF1 Peptide (AAP36895)
OPCD03993-50UGHSF1 Recombinant Protein
AAP36894-100UGHSF1 Peptide (AAP36894)
OCOA12565-20UGHSF1 Protein Lysate
OACA12489-100ULHSF1 Recombinant Antibody [5C7] (OACA12489)
OKCD06342-96WHSF1 ELISA Kit (Human)

Alternative Products

CatalogName
70R-8193HSF1 antibody

Popular Products