HSF1 Antibody

• Gene Name:

  Heat shock transcription factor 1

• Gene Aliases:

  HSTF1, Heat shock factor protein 1, Heat shock transcription factor 1, HSF 1

• Gene ID:

  15499

• Accession Number:

  NP_032322.1

• Reactivity:

  Human, Mouse, Rat, Bovine, Guinea Pig, Hamster, Monkey, Rabbit

• Immunogen:

  Purified recombinant mouse HSF1 protein, epitope mapping to amino acids 425-439

• Target:

  HSF1, or heat shock factor 1, belongs to a family of Heat Shock transcription factors that activate the transcription of genes encoding products required for protein folding, processing, targeting, degradation, and function (2) . The up-regulation of HSP (heat shock proteins) expression by stressors is achieved at the level of transcription through a heat shock element (HSE) and a transcription factor (HSF) (3, 4, 5) . Most HSFs have highly conserved amino acid sequences. On all HSFs there is a DNA binding domain at the N-terminus. Hydrophobic repeats located adjacent to this binding domain are essential for the formation of active trimers. Towards the C-terminal region another short hydrophobic repeat exists, and is thought to be necessary for suppression of trimerization (6) . There are two main heat shock factors, 1 and 2. Mouse HSF1 exists as two isoforms, however in higher eukaryotes HSF1 is found in a diffuse cytoplasmic and nuclear distribution in un-stressed cells. Once exposed to a multitude of stressors, it localizes to discrete nuclear granules within seconds. As it recovers from stress, HSF1 dissipates from these granules to a diffuse nuceloplasmic distribution. HSF2 on the other hand is similar to mouse HSF1, as it exists as two isoforms, the alpha form being more transcriptionally active than the smaller beta form (7, 8) . Various experiments have suggested that HFS2 may have roles in differentiation and development (9, 10, 11) .

• Clonality:

  Monoclonal

• Clone:

  4B4

• Conjugation:

  Unconjugated

• Type:

  Monoclonal Antibody

• Applications:

  WB, IHC, ICC, IF, IP, ELISA, GS

• Purification:

  Protein G Purified

• Assay Protocol:

  Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

• Concentration:

  1 mg/ml

• Dilution:

  WB (1:1000), IHC (1:2000), ICC/IF (1:100) ; optimal dilutions for assays should be determined by the user.

• Format:

  Liquid// PBS pH 7.4, 50% glycerol, 0.1% sodium azide

• Buffer:

  PBS pH 7.4, 50% glycerol, 0.1% sodium azide

• Reconstitution:

  Store at -20C. Shipping with Blue Ice or 4C.

• Molecular Weight:

  Detects ~85kDa (unstressed cell lysates) and ~95kDa (heat shocked cell lysates) .

• Fragment:

  IgG1

• Specificity:

  Detects ~85kDa (unstressed cell lysates) and ~95kDa (heat shocked cell lysates) .

• NCBI Gene Symbol:

  HSF1

• Host or Source:

  Rat

• Gene Name URL:

  HSF1

• CAS Number:

  9007-83-4