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MAG ELISA Kit (Human)

CAT: 0247-OKEH00439-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKEH00439-96WSize:96 Well
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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CAS Number
7732-18-5
Gene Name
Myelin associated glycoprotein
Gene Aliases
GMA; myelin-associated glycoprotein; sialic acid binding Ig-like lectin 4A; sialic acid-binding immunoglobulin-like lectin 4A; SIGLEC4A; SIGLEC-4A; S-MAG; SPG75.
Gene ID
4099
Accession Number
NP_001186145.1
Reactivity
Homo sapiens, Human
Target
Myelin-associated glycoprotein
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology MAG ELISA Kit (Human) (OKEH00439) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Human MAG has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. After washing, a biotinylated detector antibody specific for Human MAG is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Human MAG captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Urine, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
Detection Range
0.156-10ng/mL
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 69%
Sensitivity
0.036 ng/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
69 kDa
Specificity
Natural and recombinant Human Myelin-associated glycoprotein
Protein Length
626
NCBI Gene Symbol
MAG
Protein Name
P20916
Gene Name URL
MAG
Nucleotide Accession Number
NM_001199216.1

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