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SPHK2 ELISA Kit (Human)

CAT: 0247-OKEH00382-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKEH00382-96WSize:96 Well
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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CAS Number
7732-18-5
Gene Name
Sphingosine kinase 2
Gene Aliases
SK 2; SK-2; sphingosine kinase 2; SPK 2; SPK-2.
Gene ID
56848
Accession Number
NP_001191087.1
Reactivity
Homo sapiens, Human
Target
Sphingosine kinase 2
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology SPHK2 ELISA Kit (Human) (OKEH00382) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for SPHK2 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. After washing, a biotinylated detector antibody specific for SPHK2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample SPHK2 captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
Detection Range
15.6-1000U/mL
Sensitivity
7.8U/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
69 kDa
Specificity
Natural and recombinant Human Sphingosine kinase 2
Protein Length
654
NCBI Gene Symbol
SPHK2
Protein Name
Q9NRA0
Gene Name URL
SPHK2
Nucleotide Accession Number
NM_001204158.2

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