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CAT ELISA Kit (Mouse)

CAT: 0247-OKEH00329-96WSize: 96 WellDry Ice: NoHazardous: No
CAT#:0247-OKEH00329-96WSize:96 Well
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CAS Number
7732-18-5
Gene Name
Catalase
Gene Aliases
2210418N07; Ca; Cas; Cas1; Cas-1; catalase; catalase 1; Cs-; Cs-1.
Gene ID
12359
Accession Number
NP_033934.2
Reactivity
Mouse, Mus musculus
Target
Catalase
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Competitive
Assay Principle
Aviva Systems Biology CAT ELISA Kit (Mouse) (OKEH00329) is based on a competitive enzyme immunoassay technique. The microtiter well-plate in this kit has been pre-coated with an anti-CAT antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated CAT and incubated. The CAT found in the sample or standards competes with the biotinylated CAT for limited binding sites on the immobilized anti- CAT antibody. Excess unbound biotinylated CAT and sample or standard CAT is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated CAT captured in the well and inversely proportional to the amount of CAT which was contained in the sample or standard.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
Detection Range
15.6-1000pg/mL
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 103%
Sensitivity
7.87 pg/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
60 kDa
Notes
Formerly GWB-MCIAMZ
Specificity
Natural and recombinant Mouse Catalase
Protein Length
527
NCBI Gene Symbol
Cat
Protein Name
P24270
Gene Name URL
Cat
Nucleotide Accession Number
NM_009804.2

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