CAT ELISA Kit (Human)

CAT: 0247-OKEH00328-96WSize: 96 WellDry Ice: NoHazardous: No
CAT#:0247-OKEH00328-96WSize:96 Well
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CAS Number
7732-18-5
Gene Name
Catalase
Gene Aliases
Catalase; epididymis secretory sperm binding protein.
Gene ID
847
Accession Number
NP_001743.1
Reactivity
Homo sapiens, Human
Target
Catalase
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology CAT ELISA Kit (Human) (OKEH00328) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for CAT has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. After washing, a biotinylated detector antibody specific for CAT is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample CAT captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
Detection Range
0.312-20ng/mL
Sensitivity
0.085 ng/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
60 kDa
Specificity
Natural and recombinant Human Catalase
Protein Length
527
NCBI Gene Symbol
CAT
Protein Name
P04040
Gene Name URL
CAT
Nucleotide Accession Number
NM_001752.3

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