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BDH1 ELISA Kit (Human)

CAT: 0247-OKEH00272-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKEH00272-96WSize:96 Well
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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CAS Number
7732-18-5
Gene Name
3-hydroxybutyrate dehydrogenase 1
Gene Aliases
(R) -3-hydroxybutyrate dehydrogenase;3-hydroxybutyrate dehydrogenase;3-hydroxybutyrate dehydrogenase (heart, mitochondrial) ;3-hydroxybutyrate dehydrogenase, type 1; BDH; D-beta-hydroxybutyrate dehydrogenase, mitochondrial; epididymis secretory sperm binding protein; SDR9C1; short chain dehydrogenase/reductase family 9C member 1.
Gene ID
622
Accession Number
NP_004042.1
Reactivity
Homo sapiens, Human
Target
D-beta-hydroxybutyrate dehydrogenase, mitochondrial
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology BDH1 ELISA Kit (Human) (OKEH00272) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for BDH1 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. After washing, a biotinylated detector antibody specific for BDH1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample BDH1 captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
Detection Range
31.2-2000pg/ml
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 98%
Sensitivity
10.2 pg/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
38 kDa
Specificity
Natural and recombinant Human D-beta-hydroxybutyrate dehydrogenase, mitochondrial
Protein Length
343
NCBI Gene Symbol
BDH1
Protein Name
Q02338
Gene Name URL
BDH1
Nucleotide Accession Number
NM_004051.4

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