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CTGF ELISA Kit (Mouse)

CAT: 0247-OKEH00019-96WSize: 96 TestsDry Ice: NoHazardous: No
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CAT#:0247-OKEH00019-96WSize:96 Tests
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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CAS Number
7732-18-5
Gene Name
Cellular communication network factor 2
Gene Aliases
Ccn; CCN family member 2; cellular communication network factor 1; Connective tissue growth factor precursor (CTGF) (FISP-12 protein) (Hypertrophic chondrocyte-specific protein 24) ; Ctgf; fibroblast inducible secreated protein; fibroblast inducible secreted protein; Fis; fisp-12; Fisp12; Hcs2; Hcs24; hypertrophic chondrocyte-specific gene product 24; hypertrophic chondrocyte-specific protein 24; Protein FISP-12.
Gene ID
14219
Accession Number
NP_034347
Reactivity
Mouse, Mus musculus
Target
Connective tissue growth factor
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology CTGF ELISA Kit (Mouse) (OKEH00019) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for CTGF has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for CTGF is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample CTGF captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids
Detection Range
15.6-1000pg/mL
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 103%
Sensitivity
7.86 pg/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
38 kDa
Specificity
Natural and recombinant Mouse Connective tissue growth factor
Protein Length
348
NCBI Gene Symbol
Ccn2
Protein Name
P29268
Gene Name URL
Ccn2
Nucleotide Accession Number
NM_010217

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