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SLAMF7 ELISA Kit (Human)

CAT: 0247-OKDD00530-96WSize: 96 TestsDry Ice: NoHazardous: No
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CAT#:0247-OKDD00530-96WSize:96 Tests
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Reproducibility
Intra-assay Precision (Precision within an assay) : 3 samples with low, middle and high level SLAMF7 were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays) : 3 samples with low, middle and high level SLAMF7 were tested on 3 different plates, 8 replicates in each plate. Intra-Assay: CV_x005F Inter-Assay: CV_x005F _x005F Inter-Assay: CV_x005F Inter-Assay: CV_x005F _x005F
CAS Number
7732-18-5
Gene Name
SLAM family member 7
Gene Aliases
19A;19A24 protein; CD2 subset 1; CD2-like receptor activating cytotoxic cells; CD2-like receptor-activating cytotoxic cells; CD319; CRACC; CS1; membrane protein FOAP-12; Novel Ly9; novel LY9 (lymphocyte antigen 9) like protein; protein 19A; SLAM family member 7.
Gene ID
57823
Accession Number
NP_001269517.1
Reactivity
Homo sapiens|Human
Target
Self-ligand receptor of the signaling lymphocytic activation molecule (SLAM) family. SLAM receptors triggered by homo- or heterotypic cell-cell interactions are modulating the activation and differentiation of a wide variety of immune cells and thus are involved in the regulation and interconnection of both innate and adaptive immune response. Activities are controlled by presence or absence of small cytoplasmic adapter proteins, SH2D1A/SAP and/or SH2D1B/EAT-2. Isoform 1 mediates NK cell activation through a SH2D1A-independent extracellular signal-regulated ERK-mediated pathway (PubMed:11698418) . Positively regulates NK cell functions by a mechanism dependent on phosphorylated SH2D1B. Downstream signaling implicates PLCG1, PLCG2 and PI3K (PubMed:16339536) . In addition to heterotypic NK cells-target cells interactions also homotypic interactions between NK cells may contribute to activation. However, in the absence of SH2D1B, inhibits NK cell function. Acts also inhibitory in T-cells (By similarity) . May play a role in lymphocyte adhesion (PubMed:11802771) . In LPS-activated monocytes negatively regulates production of proinflammatory cytokines (PubMed:23695528) .
Type
ELISA Kit
Applications
Enzyme-linked immunosorbent assay
Assay Principle
Aviva Systems Biology SLAMF7 ELISA Kit (Human) (OKDD00530) is based on standard sandwich enzyme-linked immuno-sorbent assay methodology. An antibody for SLAMF7 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) . Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for SLAMF7 is added, incubated and removed. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample SLAMF7 captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, plasma, tissue homogenates, cell lysates
Detection Range
0.156-10ng/mL
Sensitivity
< 0.053 ng/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
37 kDa
Notes
Formerly GWB-KBBJB6
Specificity
This assay has high sensitivity and excellent specificity for detection of SLAMF7._x005F No significant cross-reactivity or interference between SLAMF7 and analogues was observed._x005F
Protein Length
335
NCBI Gene Symbol
SLAMF7
Protein Name
SLAM family member 7
Gene Name URL
SLAMF7
Nucleotide Accession Number
NM_001282588.1

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