PLOD1 ELISA Kit (Human)

CAT: 0247-OKDD00473-96WSize: 96 TestsDry Ice: NoHazardous: No
CAT#:0247-OKDD00473-96WSize:96 Tests
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Reproducibility
Intra-assay Precision (Precision within an assay) : 3 samples with low, middle and high level PLOD1 were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays) : 3 samples with low, middle and high level PLOD1 were tested on 3 different plates, 8 replicates in each plate. Intra-Assay: CV Inter-Assay: CV Inter-Assay: CV Inter-Assay: CV
CAS Number
7732-18-5
Gene Name
Procollagen-lysine,2-oxoglutarate 5-dioxygenase 1
Gene Aliases
EDS6; EDSKCL1; LH; LH1; LLH; lysine hydroxylase; lysyl hydroxlase 1; Lysyl hydroxylase 1; PLOD; procollagen-lysine 1, 2-oxoglutarate 5-dioxygenase 1; procollagen-lysine,2-oxoglutarate 5-dioxygenase 1.
Gene ID
5351
Accession Number
NP_000293.2
Reactivity
Homo sapiens|Human
Target
Part of a complex composed of PLOD1, P3H3 and P3H4 that catalyzes hydroxylation of lysine residues in collagen alpha chains and is required for normal assembly and cross-linkling of collagen fibrils (By similarity) . Forms hydroxylysine residues in -Xaa-Lys-Gly- sequences in collagens (PubMed:8621606, PubMed:10686424, PubMed:15854030) . These hydroxylysines serve as sites of attachment for carbohydrate units and are essential for the stability of the intermolecular collagen cross-links (Probable) .
Type
ELISA Kit
Applications
Enzyme-linked immunosorbent assay
Assay Principle
Aviva Systems Biology PLOD1 ELISA Kit (Human) (OKDD00473) is based on standard sandwich enzyme-linked immuno-sorbent assay methodology. An antibody for PLOD1 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) . Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for PLOD1 is added, incubated and removed. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample PLOD1 captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue homogenates, Cell lysates
Detection Range
0.156-10ng/mL
Sensitivity
< 0.066 ng/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
84 kDa
Notes
Formerly GWB-KBBES8
Specificity
This assay has high sensitivity and excellent specificity for detection of PLOD1. No significant cross-reactivity or interference between PLOD1 and analogues was observed.
Protein Length
727
NCBI Gene Symbol
PLOD1
Protein Name
Procollagen-lysine,2-oxoglutarate 5-dioxygenase 1
Gene Name URL
PLOD1
Nucleotide Accession Number
NM_000302.3

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