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MT3 ELISA Kit (Human)

CAT: 0247-OKDD00405-96WSize: 96 TestsDry Ice: NoHazardous: No
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CAT#:0247-OKDD00405-96WSize:96 Tests
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Reproducibility
Intra-assay Precision (Precision within an assay) : 3 samples with low, middle and high level MT3 were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays) : 3 samples with low, middle and high level MT3 were tested on 3 different plates, 8 replicates in each plate. Intra-Assay: CV Inter-Assay: CV Inter-Assay: CV Inter-Assay: CV
CAS Number
7732-18-5
Gene Name
Metallothionein 3
Gene Aliases
GIF; GIFB; GRIF; growth inhibitory factor; metallothionein 3 (growth inhibitory factor (neurotrophic) ) ; metallothionein-3; metallothionein-III; ZnMT3.
Gene ID
4504
Accession Number
NP_005945.1
Reactivity
Homo sapiens|Human
Target
Binds heavy metals. Contains three zinc and three copper atoms per polypeptide chain and only a negligible amount of cadmium. Inhibits survival and neurite formation of cortical neurons in vitro.
Type
ELISA Kit
Applications
Enzyme-linked immunosorbent assay
Assay Principle
Aviva Systems Biology MT3 ELISA Kit (Human) (OKDD00405) is based on standard sandwich enzyme-linked immuno-sorbent assay methodology. An antibody for MT3 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) . Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for MT3 is added, incubated and removed. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample MT3 captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue homogenates
Detection Range
0.156-10ng/mL
Sensitivity
< 0.054 ng/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
7 kDa
Notes
Formerly GWB-KBBAN6
Specificity
This assay has high sensitivity and excellent specificity for detection of MT3. No significant cross-reactivity or interference between MT3 and analogues was observed.
Protein Length
68
NCBI Gene Symbol
MT3
Protein Name
Metallothionein-3
Gene Name URL
MT3
Nucleotide Accession Number
NM_005954.2