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C1qL1 ELISA Kit (Human)

CAT: 0247-OKDD00157-96WSize: 96 TestsDry Ice: NoHazardous: No
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CAT#:0247-OKDD00157-96WSize:96 Tests
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Reproducibility
Intra-assay Precision (Precision within an assay) : 3 samples with low, middle and high level C1qL1 were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays) : 3 samples with low, middle and high level C1qL1 were tested on 3 different plates, 8 replicates in each plate. Intra-Assay: CV Inter-Assay: CV Inter-Assay: CV Inter-Assay: CV
CAS Number
7732-18-5
Gene Name
Complement C1q like 1
Gene Aliases
C1q and tumor necrosis factor-related protein 14; C1q/TNF-related protein 14; C1q-related factor; C1QRF; C1QTNF14; Complement component 1 Q subcomponent-like 1; complement component 1, q subcomponent-like 1; CRF; CTRP14.
Gene ID
10882
Accession Number
NP_006679.1
Reactivity
Homo sapiens|Human
Target
May regulate the number of excitatory synapses that are formed on hippocampus neurons. Has no effect on inhibitory synapses (By similarity) .
Type
ELISA Kit
Applications
Enzyme-linked immunosorbent assay
Assay Principle
Aviva Systems Biology C1qL1 ELISA Kit (Human) (OKDD00157) is based on standard sandwich enzyme-linked immuno-sorbent assay methodology. An antibody for C1qL1 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) . Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for C1qL1 is added, incubated and removed. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample C1qL1 captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue homogenates
Detection Range
0.156-10ng/mL
Sensitivity
< 0.053 ng/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
26 kDa
Notes
Formerly GWB-KBBZF6
Specificity
This assay has high sensitivity and excellent specificity for detection of C1qL1. No significant cross-reactivity or interference between C1qL1 and analogues was observed.
Protein Length
258
NCBI Gene Symbol
C1QL1
Protein Name
C1q-related factor
Gene Name URL
C1QL1
Nucleotide Accession Number
NM_006688.4

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