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C1QL3 ELISA Kit (Human)

CAT: 0247-OKCA00643-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKCA00643-96WSize:96 Well
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Reproducibility
Inter-Assay CV:
CAS Number
7732-18-5
Gene Name
Complement C1q like 3
Gene Aliases
C1ql, K100, CTRP13, C1QTNF13
Gene ID
389941
Accession Number
NP_001010908.1
Reactivity
Human
Target
May regulate the number of excitatory synapses that are formed on hippocampus neurons. Has no effect on inhibitory synapses. Plays a role in glucose homeostasis. Via AMPK signaling pathway, stimulates glucose uptake in adipocytes, myotubes and hepatocytes and enhances insulin-stimulated glucose uptake. In a hepatoma cell line, reduces the expression of gluconeogenic enzymes G6PC and PCK1 and hence decreases de novo glucose production.
Type
ELISA Kit
Assay Principle
Aviva Systems Biology C1QL3 ELISA Kit (Human) (OKCA00643) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for C1QL3 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) . Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for C1QL3 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample C1QL3 captured in the well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates
Detection Range
0.312 ng/mL-20 ng/mL
Recovery
Matrix Recovery range (%) Average (%) Serum (n=5) 95 90-100 EDTA Plasma (n=4) 90 85-95 Matrix Recovery range (%) Average (%) Matrix Recovery range (%) Average (%) Serum (n=5) 95 90-100 Serum (n=5) 95 90-100 EDTA Plasma (n=4) 90 85-95 EDTA Plasma (n=4) 90 85-95
Sensitivity
0.078 ng/mL
Reconstitution
Store as indicated in product manual.
NCBI Gene Symbol
C1QL3
Protein Name
Complement C1q-like protein 3
Gene Name URL
C1QL3
Nucleotide Accession Number
NM_001010908.1

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