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AIMP1 ELISA Kit (Mouse)

CAT: 0247-OKCD09321-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKCD09321-96WSize:96 Well
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Reproducibility
Intra-assay Precision (Precision within an assay) : 3 samples with low, middle and high level Aminoacyl tRNA Synthetase Complex Interacting Multifunctional Protein 1 (AIMP1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays) : 3 samples with low, middle and high level Aminoacyl tRNA Synthetase Complex Interacting Multifunctional Protein 1 (AIMP1) were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
CAS Number
7732-18-5
Gene Aliases
Multisynthase complex auxiliary component p43.
Reactivity
Mouse|Mus musculus
Target
Non-catalytic component of the multisynthase complex (PubMed:12060739) . Stimulates the catalytic activity of cytoplasmic arginyl-tRNA synthase (By similarity) . Binds tRNA. Possesses inflammatory cytokine activity (PubMed:1400342, PubMed:7545917) . Negatively regulates TGF-beta signaling through stabilization of SMURF2 by binding to SMURF2 and inhibiting its SMAD7-mediated degradation (PubMed:18448069) . Involved in glucose homeostasis through induction of glucagon secretion at low glucose levels (PubMed:17001013) . Promotes dermal fibroblast proliferation and wound repair (PubMed:15681823) . Regulates KDELR1-mediated retention of HSP90B1/gp96 in the endoplasmic reticulum (PubMed:17525271) . Plays a role in angiogenesis by inducing endothelial cell migration at low concentrations and endothelian cell apoptosis at high concentrations (By similarity) . Induces maturation of dendritic cells and monocyte cell adhesion (PubMed:18292511) . Modulates endothelial cell responses by degrading HIF-1A through interaction with PSMA7 (By similarity) .
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Detection Method
Colorimetric
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Aminoacyl tRNA Synthetase Complex Interacting Multifunctional Protein 1 (AIMP1) . Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Aminoacyl tRNA Synthetase Complex Interacting Multifunctional Protein 1 (AIMP1) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Aminoacyl tRNA Synthetase Complex Interacting Multifunctional Protein 1 (AIMP1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Aminoacyl tRNA Synthetase Complex Interacting Multifunctional Protein 1 (AIMP1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, plasma, tissue homogenates and other biological fluids
Detection Range
78-5,000pg/mL
Sensitivity
< 11.3pg/mL
Reconstitution
2°C to 8°C|-20°C
Components
Component Amount AIMP1 Microplate 96 Wells (12 x 8 Well strips) AIMP1 Lyophilized Standard 2 100X AIMP1 HRP-Detector Anitbody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL TMB Substrate 1 x 9 mL Stop Solution 1 x 6 mL Component Amount Component Amount AIMP1 Microplate 96 Wells (12 x 8 Well strips) AIMP1 Microplate 96 Wells (12 x 8 Well strips) AIMP1 Lyophilized Standard 2 AIMP1 Lyophilized Standard 2 100X AIMP1 HRP-Detector Anitbody 1 x 120 uL 100X AIMP1 HRP-Detector Anitbody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL 30X Wash Buffer 1 x 20 mL TMB Substrate 1 x 9 mL TMB Substrate 1 x 9 mL Stop Solution 1 x 6 mL Stop Solution 1 x 6 mL
NCBI Gene Symbol
AIMP1
Protein Name
Aminoacyl tRNA synthase complex-interacting multifunctional protein 1
Gene Name URL
AIMP1

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