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ELOVL1 ELISA Kit (Human)

CAT: 0247-OKCD09153-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKCD09153-96WSize:96 Well
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Reproducibility
Intra-assay Precision (Precision within an assay) : 3 samples with low, middle and high level Elongation Of Very Long Chain Fatty Acids Like Protein 1 (ELOVL1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays) : 3 samples with low, middle and high level Elongation Of Very Long Chain Fatty Acids Like Protein 1 (ELOVL1) were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
CAS Number
7732-18-5
Gene Name
ELOVL fatty acid elongase 1
Gene Aliases
3-keto acyl-CoA synthase ELOVL1; CGI-88; elongation of very long chain fatty acids (FEN1/Elo2, SUR4/Elo3, yeast) -like 1; elongation of very long chain fatty acids protein 1; ELOVL FA elongase 1; ELOVL fatty acid elongase 1; IKSHD; Ssc1; very long chain 3-ketoacyl-CoA synthase 1; very long chain 3-oxoacyl-CoA synthase 1.
Gene ID
64834
Accession Number
NP_001243328.1
Reactivity
Homo sapiens|Human
Target
Catalyzes the first and rate-limiting reaction of the four that constitute the long-chain fatty acids elongation cycle. This endoplasmic reticulum-bound enzymatic process, allows the addition of 2 carbons to the chain of long- and very long-chain fatty acids/VLCFAs per cycle. Condensing enzyme that exhibits activity toward saturated C18 to C26 acyl-CoA substrates, with the highest activity towards C22:0 acyl-CoA. May participate in the production of both saturated and monounsaturated VLCFAs of different chain lengths that are involved in multiple biological processes as precursors of membrane lipids and lipid mediators. Important for saturated C24:0 and monounsaturated C24:1 sphingolipid synthesis. Indirectly inhibits RPE65 via production of VLCFAs.
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Detection Method
Colorimetric
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Elongation Of Very Long Chain Fatty Acids Like Protein 1 (ELOVL1) . Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Elongation Of Very Long Chain Fatty Acids Like Protein 1 (ELOVL1) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Elongation Of Very Long Chain Fatty Acids Like Protein 1 (ELOVL1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Elongation Of Very Long Chain Fatty Acids Like Protein 1 (ELOVL1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Tissue homogenates, cell lysates and other biological fluids.
Detection Range
0.156-10ng/mL
Sensitivity
< 0.062ng/mL
Reconstitution
2°C to 8°C|-20°C
Components
Component Amount ELOVL1 Microplate 96 Wells (12 x 8 Well strips) ELOVL1 Lyophilized Standard 2 100X ELOVL1 HRP-Detector Anitbody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL TMB Substrate 1 x 9 mL Stop Solution 1 x 6 mL Component Amount Component Amount ELOVL1 Microplate 96 Wells (12 x 8 Well strips) ELOVL1 Microplate 96 Wells (12 x 8 Well strips) ELOVL1 Lyophilized Standard 2 ELOVL1 Lyophilized Standard 2 100X ELOVL1 HRP-Detector Anitbody 1 x 120 uL 100X ELOVL1 HRP-Detector Anitbody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL 30X Wash Buffer 1 x 20 mL TMB Substrate 1 x 9 mL TMB Substrate 1 x 9 mL Stop Solution 1 x 6 mL Stop Solution 1 x 6 mL
NCBI Gene Symbol
ELOVL1
Protein Name
Elongation of very long chain fatty acids protein 1
Gene Name URL
ELOVL1
Nucleotide Accession Number
NM_001256399.1

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