Products for Research Use Only
RegisterLogin

P2RX7 ELISA Kit (Human)

CAT: 0247-OKCD08890-96WSize: 96 WellDry Ice: NoHazardous: No
Product image 1
1 / 1
CAT#:0247-OKCD08890-96WSize:96 Well
Selected
24/48H Stock Items & 2 to 6 Weeks non Stock Items.
Quick Request Actions
Reproducibility
Intra-assay Precision (Precision within an assay) : 3 samples with low, middle and high level Purinergic Receptor P2X, Ligand Gated Ion Channel 7 (P2RX7) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays) : 3 samples with low, middle and high level Purinergic Receptor P2X, Ligand Gated Ion Channel 7 (P2RX7) were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
CAS Number
7732-18-5
Gene Name
Purinergic receptor P2X 7
Gene Aliases
ATP receptor; P2X purinoceptor 7; P2X7; P2X7 receptor; P2Z receptor; Purinergic receptor; purinergic receptor P2X, ligand gated ion channel, 7; purinergic receptor P2X7 variant A; purnergic receptor P2X 7.
Gene ID
5027
Accession Number
NP_002553.3
Reactivity
Homo sapiens|Human
Target
Receptor for ATP that acts as a ligand-gated ion channel. Responsible for ATP-dependent lysis of macrophages through the formation of membrane pores permeable to large molecules. Could function in both fast synaptic transmission and the ATP-mediated lysis of antigen-presenting cells. In the absence of its natural ligand, ATP, functions as a scavenger receptor in the recognition and engulfment of apoptotic cells (PubMed:21821797, PubMed:23303206) .
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Detection Method
Colorimetric
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Purinergic Receptor P2X, Ligand Gated Ion Channel 7 (P2RX7) . Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Purinergic Receptor P2X, Ligand Gated Ion Channel 7 (P2RX7) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Purinergic Receptor P2X, Ligand Gated Ion Channel 7 (P2RX7), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Purinergic Receptor P2X, Ligand Gated Ion Channel 7 (P2RX7) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Tissue homogenates, cell lysates and other biological fluids
Detection Range
0.156-10ng/mL
Sensitivity
< 0.059ng/mL
Reconstitution
2°C to 8°C|-20°C
Components
Component Amount P2RX7 Microplate 96 Wells (12 x 8 Well strips) P2RX7 Lyophilized Standard 2 100X P2RX7 HRP-Detector Anitbody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL TMB Substrate 1 x 2 mL Stop Solution 1 x 20 mL Component Amount Component Amount P2RX7 Microplate 96 Wells (12 x 8 Well strips) P2RX7 Microplate 96 Wells (12 x 8 Well strips) P2RX7 Lyophilized Standard 2 P2RX7 Lyophilized Standard 2 100X P2RX7 HRP-Detector Anitbody 1 x 120 uL 100X P2RX7 HRP-Detector Anitbody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL 30X Wash Buffer 1 x 20 mL TMB Substrate 1 x 2 mL TMB Substrate 1 x 2 mL Stop Solution 1 x 20 mL Stop Solution 1 x 20 mL
NCBI Gene Symbol
P2RX7
Protein Name
P2X purinoceptor 7
Gene Name URL
P2RX7
Nucleotide Accession Number
NM_002562.5

Related Products

CatalogName
OKEH05605-96WP2RX7 ELISA Kit (Mouse)
OKCD03848-96WP2RX7 Chemi-Luminescent ELISA Kit (Human)
OKEH05605P2RX7 ELISA Kit (Mouse) (OKEH05605)
OKCD03848P2RX7 Chemi-Luminescent ELISA Kit (Human) (OKCD03848)
OASA05732-250UGP2rx7 Antibody
OAGA03764-100ULP2RX7 Antibody
ARP35518_T100P2RX7 (ARP35518_T100)
OASA05733-100UGP2rx7 Antibody
AWBK35518P2RX7 Western Blot kit (AWBK35518)
OPCD05941-200UGP2RX7 Recombinant Protein

Alternative Products

CatalogName
E032322Human P2RX7 ELISA Kit
GTR18830970-01Human P2RX7 ELISA Kit (Ready to Use)
MBS9902686-01Human P2X Purinoceptor 7 (P2RX7) ELISA Kit
E1850HuHuman P2RX7/P2X purinoceptor 7 ELISA Kit
E5825Hu-01Human P2X purinoceptor 7, P2RX7 ELISA KIT
F6437P2X purinoceptor 7 (P2RX7) Human ELISA Kit
ELI-45176hHuman P2X purinoceptor 7,P2RX7 ELISA KIT
KBH14251GENLISA Human P2X Purinoceptor 7 (P2RX7) ELISA
EH1408Human P2RX7 (P2X purinoceptor 7) ELISA Kit
KBH5825GENLISA™ Human P2X purinoceptor 7 (P2RX7) ELISA

Popular Products