SLIT3 ELISA Kit (Human)

CAT: 0247-OKCD08461-96WSize: 96 WellDry Ice: NoHazardous: No
CAT#:0247-OKCD08461-96WSize:96 Well
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Reproducibility
Intra-assay Precision (Precision within an assay) : 3 samples with low, middle and high level Slit Homolog 3 (Slit3) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays) : 3 samples with low, middle and high level Slit Homolog 3 (Slit3) were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
CAS Number
7732-18-5
Gene Name
Slit guidance ligand 3
Gene Aliases
MEGF5; multiple EGF-like domains protein 5; multiple epidermal growth factor-like domains protein 5; SLIL2; slit homolog 3; slit homolog 3 protein; SLIT1; slit2; Slit-3.
Gene ID
6586
Accession Number
NP_001258875.1
Reactivity
Homo sapiens|Human
Target
May act as molecular guidance cue in cellular migration, and function may be mediated by interaction with roundabout homolog receptors.
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Detection Method
Colorimetric
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Slit Homolog 3 (Slit3) . Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Slit Homolog 3 (Slit3) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Slit Homolog 3 (Slit3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Slit Homolog 3 (Slit3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Detection Range
0.156-10ng/mL
Sensitivity
< 0.059ng/mL
Reconstitution
2°C to 8°C|-20°C
Components
Component Amount SLIT3 Microplate 96 Wells (12 x 8 Well strips) SLIT3 Lyophilized Standard 2 100X SLIT3 HRP-Detector Anitbody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL TMB Substrate 1 x 9 mL Stop Solution 1 x 6 mL Component Amount Component Amount SLIT3 Microplate 96 Wells (12 x 8 Well strips) SLIT3 Microplate 96 Wells (12 x 8 Well strips) SLIT3 Lyophilized Standard 2 SLIT3 Lyophilized Standard 2 100X SLIT3 HRP-Detector Anitbody 1 x 120 uL 100X SLIT3 HRP-Detector Anitbody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL 30X Wash Buffer 1 x 20 mL TMB Substrate 1 x 9 mL TMB Substrate 1 x 9 mL Stop Solution 1 x 6 mL Stop Solution 1 x 6 mL
NCBI Gene Symbol
SLIT3
Protein Name
Slit homolog 3 protein
Gene Name URL
SLIT3
Nucleotide Accession Number
NM_001271946.1

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