Products for Research Use Only
RegisterLogin

LDLRAP1 ELISA Kit (Mouse)

CAT: 0247-OKCD08387-96WSize: 96 WellDry Ice: NoHazardous: No
Product image 1
1 / 1
CAT#:0247-OKCD08387-96WSize:96 Well
Selected
24/48H Stock Items & 2 to 6 Weeks non Stock Items.
Quick Request Actions
Reproducibility
Intra-assay Precision (Precision within an assay) : 3 samples with low, middle and high level Low Density Lipoprotein Receptor Adaptor Protein 1 (LDLRAP1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays) : 3 samples with low, middle and high level Low Density Lipoprotein Receptor Adaptor Protein 1 (LDLRAP1) were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
CAS Number
7732-18-5
Gene Name
Low density lipoprotein receptor adaptor protein 1
Gene Aliases
A; AA691260; Ar; Arh; Arh1; ARH2; autosomal recessive hypercholesterolemia protein homolog; FHCB1; FHCB2; LDL receptor adaptor protein; low density lipoprotein receptor adapter protein 1.
Gene ID
100017
Accession Number
NP_663529.2
Reactivity
Mouse|Mus musculus
Target
Adapter protein (clathrin-associated sorting protein (CLASP) ) required for efficient endocytosis of the LDL receptor (LDLR) in polarized cells such as hepatocytes and lymphocytes, but not in non-polarized cells (fibroblasts) . May be required for LDL binding and internalization but not for receptor clustering in coated pits. May facilitate the endocytocis of LDLR and LDLR-LDL complexes from coated pits by stabilizing the interaction between the receptor and the structural components of the pits. May also be involved in the internalization of other LDLR family members. Binds to phosphoinositides, which regulate clathrin bud assembly at the cell surface. Required for trafficking of LRP2 to the endocytic recycling compartment which is necessary for LRP2 proteolysis, releasing a tail fragment which translocates to the nucleus and mediates transcriptional repression (By similarity) .
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Detection Method
Colorimetric
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Low Density Lipoprotein Receptor Adaptor Protein 1 (LDLRAP1) . Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Low Density Lipoprotein Receptor Adaptor Protein 1 (LDLRAP1) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Low Density Lipoprotein Receptor Adaptor Protein 1 (LDLRAP1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Low Density Lipoprotein Receptor Adaptor Protein 1 (LDLRAP1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Tissue homogenates and other biological fluids.
Detection Range
0.156-10ng/mL
Sensitivity
< 0.067ng/mL
Reconstitution
2°C to 8°C|-20°C
Components
Component Amount LDLRAP1 Microplate 96 Wells (12 x 8 Well strips) LDLRAP1 Lyophilized Standard 2 100X LDLRAP1 HRP-Detector Anitbody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL TMB Substrate 1 x 9 mL Stop Solution 1 x 6 mL Component Amount Component Amount LDLRAP1 Microplate 96 Wells (12 x 8 Well strips) LDLRAP1 Microplate 96 Wells (12 x 8 Well strips) LDLRAP1 Lyophilized Standard 2 LDLRAP1 Lyophilized Standard 2 100X LDLRAP1 HRP-Detector Anitbody 1 x 120 uL 100X LDLRAP1 HRP-Detector Anitbody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL 30X Wash Buffer 1 x 20 mL TMB Substrate 1 x 9 mL TMB Substrate 1 x 9 mL Stop Solution 1 x 6 mL Stop Solution 1 x 6 mL
NCBI Gene Symbol
Ldlrap1
Protein Name
Low density lipoprotein receptor adapter protein 1
Gene Name URL
Ldlrap1
Nucleotide Accession Number
NM_145554.2

Related Products

CatalogName
OKCA00714LDLRAP1 ELISA Kit (Human) (OKCA00714)
OACA06933-100ULLDLRAP1 Antibody
OAGA05188-100ULLDLRAP1 Antibody
OAAN03635-100ULLDLRAP1 Antibody
OACA06934-50ULLDLRAP1 Antibody
OACD05204-1MLLDLRAP1 Antibody
OAAN03635-50ULLDLRAP1 Antibody
OACA06934-100ULLDLRAP1 Antibody
OACD05204-100ULLDLRAP1 Antibody
OACA06933-50ULLDLRAP1 Antibody

Alternative Products

CatalogName
GTR18827895-01Mouse LDLRAP1 ELISA Kit
GTR18832105-01Mouse LDLRAP1 ELISA Kit (Ready to Use)
AE35812MO-01Mouse Low density lipoprotein receptor adapter protein 1 (LDLRAP1) ELISA Kit
RD-LDLRAP1-Mu-01Mouse Low Density Lipoprotein Receptor Adaptor Protein 1 (LDLRAP1) ELISA Kit
E7348Low Density Lipoprotein Receptor adapter protein 1 (LDLRAP1) Mouse ELISA Kit
KTE71191-01ELISA kit for Mouse Low density lipoprotein receptor adapter protein 1 (LDLRAP1)
EM23660Mouse LDLRAP1 (Low Density Lipoprotein Receptor Adaptor Protein 1) ELISA Kit
KLM5847GENLISA™ Mouse Low Density Lipoprotein Receptor Adaptor Protein 1 (LDLRAP1) ELISA
MBS8237459-01LDLRAP1 siRNA (Mouse)
26422054LDLRAP1 Adenovirus (Mouse)

Popular Products