DRD4 ELISA Kit (Rat)

CAT: 0247-OKCD06986-96WSize: 96 WellDry Ice: NoHazardous: No
CAT#:0247-OKCD06986-96WSize:96 Well
Selected
PRICE
AVAILABILITY: InStock
24/48H Stock Items & 2 to 6 Weeks non Stock Items.
Product image 1
1 / 1
Reproducibility
Intra-assay Precision (Precision within an assay) : 3 samples with low, middle and high level Dopamine Receptor D4 (DRD4) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays) : 3 samples with low, middle and high level Dopamine Receptor D4 (DRD4) were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
CAS Number
7732-18-5
Gene Aliases
D (2C) dopamine receptor; Dopamine D4 receptor.
Reactivity
Rat|Rattus norvegicus
Target
Dopamine receptor responsible for neuronal signaling in the mesolimbic system of the brain, an area of the brain that regulates emotion and complex behavior. Activated by dopamine, but also by epinephrine and norepinephrine, and by numerous synthetic agonists and drugs (By similarity) . Agonist binding triggers signaling via G proteins that inhibit adenylyl cyclase (PubMed:10832611) . Modulates the circadian rhythm of contrast sensitivity by regulating the rhythmic expression of NPAS2 in the retinal ganglion cells (By similarity) .
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Detection Method
Colorimetric
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Dopamine Receptor D4 (DRD4) . Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Dopamine Receptor D4 (DRD4) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Dopamine Receptor D4 (DRD4), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Dopamine Receptor D4 (DRD4) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Tissue homogenates, cell lysates and other biological fluids
Detection Range
0.156-10ng/mL
Sensitivity
< 0.054ng/mL
Reconstitution
2°C to 8°C|-20°C
Components
Component Amount DRD4 Microplate 96 Wells (12 x 8 Well strips) DRD4 Lyophilized Standard 2 100X DRD4 HRP-Detector Anitbody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL TMB Substrate 1 x 2 mL Stop Solution 1 x 20 mL Component Amount Component Amount DRD4 Microplate 96 Wells (12 x 8 Well strips) DRD4 Microplate 96 Wells (12 x 8 Well strips) DRD4 Lyophilized Standard 2 DRD4 Lyophilized Standard 2 100X DRD4 HRP-Detector Anitbody 1 x 120 uL 100X DRD4 HRP-Detector Anitbody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL 30X Wash Buffer 1 x 20 mL TMB Substrate 1 x 2 mL TMB Substrate 1 x 2 mL Stop Solution 1 x 20 mL Stop Solution 1 x 20 mL
NCBI Gene Symbol
DRD4
Protein Name
D (4) dopamine receptor
Gene Name URL
DRD4

Related Products

CatalogName
OKEI00207DRD4 ELISA Kit (Human) (OKEI00207)
OKEI00443DRD4 ELISA Kit (Mouse) (OKEI00443)
OAAJ07572-100ULDRD4 Antibody
OAAJ05752-100ULDRD4 Antibody
OAAF04791-100UGDRD4 Antibody
OKEH04821-96WDrd1 ELISA Kit (Rat)
OKCD06652-96WDRD2 ELISA Kit (Rat)
OKCD07296-96WDRD1 ELISA Kit (Rat)
OKEH03182-96WDRD2 ELISA Kit (Rat)
OAAB07181-400ULDRD4 Antibody - middle region

Alternative Products

CatalogName
E081688Rat DRD4 ELISA Kit
MBS2503768-01Rat DRD4 (Dopamine Receptor D4) ELISA Kit
MBS458928-01Rat Dopamine Receptor D4 (DRD4) ELISA Kit
E-EL-R0342ELISA kit for Rat DRD4 (Dopamine Receptor D4)
BG-RAT10762Rat Dopamine Receptor D4 DRD4 ELISA Kit
CRR0674-01DRD4 siRNA (Rat)
18597056DRD4 Adenovirus (Rat)
abx914625-01Rat DRD4 siRNA
abx985021-01Rat DRD4 shRNA Plasmid
RP198671DRD4 Recombinant Protein (Rat)

Popular Products